GoTaq Probe qPCR Master Mix from Promega

Product Specs
ItemGoTaq Probe qPCR Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA6101
Quantity2ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Transrenal Mycobacterium tuberculosis DNA in pulmonary tuberculosis patients during the first 14 days of treatmentMicrobiology SpectrumDecember 12, 2023Irina Kontsevaya, Jan Heyckendorf, Frauke Koops, Doris Hillemann, Torsten Goldmann, Caryn M. Upton, Veronique De Jager, Andreas Diacon, Christoph Lange(Roche, Switzerland). Amplification mix included 1× GoTaq Probe qPCR Master Mix (Promega, USA; cat. no. A6101), 500 nM forward (5′-ACCAGCACCTAACCGGCTGT-3′) and reverse (5′-GTAGGCGAACCCTGCCCAG-3′) primers, and 200

(2) ER‐mitochondria contacts and cholesterol metabolism are disrupted by disease‐associated tau proteinEMBO reportsAugust 3, 2023Leonora Szabo, Nadia Cummins, Paolo Paganetti, Alex Odermatt, Andreas Papassotiropoulos, Celeste Karch, Jürgen Götz, Anne Eckert, Amandine GrimmRNeasy Mini Kit (250),Qiagen,74106 "GoScript™ Reverse Transcription Mix, Oligo(dT)",Promega,A2791 GoTaq® Probe qPCR Master Mix,Promega,A6102 MycoAlert® PLUS Mycoplasma Detection Kit,Lonza,LT07‐701 Oligonucleotides,Oligonucleotides,Oligonucleotides AllStars Negative Control siRNA

(3) Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periodsCell Reports MethodsJune 1, 2023Fabian Otte, Yuepeng Zhang, Julian Spagnuolo, Alexander Thielen, Martin Däumer, Carsten Wiethe, Marcel Stoeckle, Katharina Kusejko, Florian Klein, Karin J. Metzner, et al.assays ,, Maxwell® RSC Cultured Cells DNA Kit,Promega,Cat#: AS1620 GoTaq® Probe qPCR Master Mix,Promega,Cat#: A6102 Maxwell® RSC simply RNA cells kit,Promega,Cat#: AS1390 qRT-PCR Brilliant III probe master mix kit,Agilent,Cat#:

(4) Expression of dual-specificity phosphatases in TGFß1-induced EMT in SKOV3 cellsTurkish Journal of Medical SciencesFebruary 21, 2023SABİRE GÜLER, ABDULLAH YALÇINanalyses were performed on StepOne Plus (ThermoFischer, U.S.A) using gene expression master mix (Promega, A6101) and gene-specific TaqMan probes (ThermoFisher). The probes used were Snail (Hs00195591_m1), Slug (Hs00161904_m1),

(5) Precise Authenticity of Quinoa, Coix Seed, Wild Rice and Chickpea Components Using Optimized TaqMan Real-Time PCRFoodsFebruary 16, 2023Qiuyue Zheng, Xinying Yin, Aifu Yang, Ning Yu, Ranran Xing, Ying Chen, Ruijie Deng, Jijuan Caousing Premix Ex TaqTM Probe qPCR (RR390 A, TAKARA, Beijing, China) and GoTaq qPCR Master Mix (Cat. A6101, Promega, Fitchburg, WI, USA); primer and probe concentrations (−20%), reaction mix volume (2×) (±1

(6) Establish an allele-specific real-time PCR for Leishmania species identificationInfectious Diseases of PovertyDecember 1, 2022Yun Wu, Mengyuan Jiang, Shaogang Li, Nicholas R. Waterfield, Guowei Yangspecies identification, a reaction containing 10 μl of Promega GoTaq Probe qPCR Master Mix (Promega, A6101, Madison, WI, USA), 800 nmol/l forward primer VL-HGP-F2, 800 nmol/L reverse primer VL-HGP-R1, 450 nmol/L

(7) Methotrexate-Induced Liver Injury Is Associated with Oxidative Stress, Impaired Mitochondrial Respiration, and Endoplasmic Reticulum Stress In VitroInternational journal of molecular sciencesDecember 1, 2022Schmidt S, Messner CJ, Gaiser C, Hämmerli C, Suter-Dick L.4673417001; for SLC19A1) or GoTaq® Probe qPCR and RT-qPCR Systems (Promega, Dübendorf, Switzerland, A6102; for DDIT3 and ACTA2) and TaqMan probes of selected genes (see Table 2). The q-RT-PCR Program used:

(8) Inducing respiratory complex I impairment elicits an increase in PGC1α in ovarian cancerScientific ReportsDecember 1, 2022Monica De Luise et al.63 °C 45 s. The qRT–PCR with TaqMan assay was performed with GoTaq® Probe qPCR Master Mix (Promega #A6101 and #A6102) and run in the abovementioned system, using the following conditions: 95 °C 2 min; 40 cycles

(9) Codon Usage and mRNA Stability are Translational Determinants of Cellular Response to Canonical Ferroptosis InducersNeuroscienceOctober 1, 2022Sherif Rashad, Shane R Byrne, Daisuke Saigusa, Jingdong Xiang, Yuan Zhou, Liyin Zhang, Thomas J Begley, Teiji Tominaga, Kuniyasu NiizumaSuperscript III reverse transcriptase (ThermoFisher, Cat# 18080044). qPCR was performed using GoTaq qPCR Master Mix (Promega, Cat# A6102) on CFX96 thermal cycler (Bio-Rad). qPCR was conducted with 4 replicates per group. qPCR was analyzed

(10) Predictive power of SARS-CoV-2 wastewater surveillance for diverse populations across a large geographical rangemedRxivJuly 29, 2022Richard G. Melvin, Nabiha Chaudhry, Onimitein Georgewill, Rebecca Freese, Glenn E. SimmonsDNA Technologies 10006713) and Go-Taq Probe 1-Step RT-qPCR system 2X master mixes with dUTP (Promega A6102). Each 20-μl RT-qPCR contained 5μl of extracted RNA, 1.5μl primer/probe mix, 10μl Go-Taq master mix,

(11) Rapid Detection of Actinobacillus pleuropneumoniae From Clinical Samples Using Recombinase Polymerase AmplificationFrontiers in Veterinary ScienceMarch 25, 2022Oliver W. Stringer et al.(Sigma-Aldrich, UK, Cat #VC0023N) and 1x GoTaq probe qPCR master mix (Promega, Southampton, UK, Cat #A6102) for a total reaction volume of 5 μL. Reactions were run in a Bio-Rad CFX Connect Real-Time PCR thermocycler

(12) Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assayPLOS Neglected Tropical DiseasesDecember 17, 2021Yun Wu, Fei Wang, Chaoyue Wang, Xinming Tang, Xianyong Liu, Shaogang Li, Nicholas R. Waterfield, Wei Wang, Xun Suo, Guowei Yangjirovecii; TG-Probe (5′ Texas Red/3′ BHQ2) for T. gondii with 10μl GoTaq Probe qPCR Master Mix (Promega, A6101, Madison, WI, USA) plus 1μl template DNA (5–50 ng). The reaction was performed in the Applied Biosystems

(13) Deoxyribonuclease 1 Q222R single nucleotide polymorphism and long-term mortality after acute myocardial infarctionBasic Research in CardiologyDecember 1, 2021Thomas M. Hofbauer et al.TaqMan™ allelic discrimination assay (ThermoFisher, 4351379) and GoTaq® Probe qPCR master mix (Promega, A6102) were used according to manufacturer’s instructions and performed in duplicates. Fluorescence was measured,

(14) A novel wastewater-based epidemiology indexing method predicts SARS-CoV-2 disease prevalence across treatment facilities in metropolitan and regional populationsScientific ReportsDecember 1, 2021Richard G. Melvin, Emily N. Hendrickson, Nabiha Chaudhry, Onimitein Georgewill, Rebecca Freese, Timothy W. Schacker, Glenn E. SimmonsDNA Technologies 10006713) and Go-Taq Probe 1-Step qRT-PCR system 2X master mixes with dUTP (Promega A6102). Each 20-µL qRT-PCR contained 5 µL of extracted RNA, 1.5 µL primer/probe mix, 10 µL Go-Taq master mix,

(15) Apoptosis Enhances the Replication of Human Coronavirus OC43VirusesNovember 1, 2021Maharjan S, Kang M, Kim J, Kim D, Park S, Kim M, Baek K, Lee Y, Kwon HJ.were synthesized by Genotech (Daejeon, South Korea). GoTaq® Probe qPCR Master Mix (10 µL) (Catalog No. A6101, Promega, Madison, WI, USA) was added to 10 µL reaction mixture containing forward and reverse primers

(16) Metagenomic Sequencing for Identification of Xylella fastidiosa from Leaf SamplesmSystemsOctober 26, 2021Verónica Román-Reyna et al.following the multilocus sequence typing (MLST) approach (36). The GoTaq probe qPCR master mix (Promega; A6102) and GoTaq G2 (Promega; M784B) polymerase were used for qPCR and conventional PCR experiments, respectively.

(17) The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8Cell researchSeptember 1, 2021Zhang JY, Zhou B, Sun RY, Ai YL, Cheng K, Li FN, Wang BR, Liu FJ, Jiang ZH, Wang WJ, et al.Biotech. CytoTox 96 nonradioactive cytotoxicity assay kit (Cat# G1780) and GoTaq qPCR master mix (Cat# A6101) were purchased from Promega. Lactic acid (Cat# 50-21-5) and hydrogen peroxide (Cat# 7722-84-1) were

(18) Lack of active SARS-CoV-2 virus in a subset of PCR-positive COVID-19 congregate care patientsJournal of Clinical VirologyAugust 1, 2021Amit K. Singh, Kathleen A. Stellrecht, Thilaka Arunachalam, Tarani K. Barman, Michael D. Robek, Michael J. Waxman, Sarah L. Elmendorf, Dennis W. Metzgerinstructions. RT-qPCR quantification of viral titers used a single step qPCR kit (Promega catalog #A6101) with primers designed to amplify N1 and N2 nucleic acids, and probes procured from IDT, USA. A known

(19) A mid-throughput HBV replication inhibition assay capable of detecting ribonuclease H inhibitorsJournal of Virological MethodsJune 1, 2021Qilan Li, Tiffany C. Edwards, Nathan L. Ponzar, John E. TavisNo amplification from a purified DNA template was observed when the GoTaq qPCR master mix (Promega A6101) used for the 12-well assay was tested. We next tested Kappa Probe Force master mix (Roche KK4303) and

(20) Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells upon SARS-CoV-2 InfectionBiomolecules & TherapeuticsMay 1, 2021Byoung Kwon Park, Dongbum Kim, Sangkyu Park, Sony Maharjan, Jinsoo Kim, Jun-Kyu Choi, Madhav Akauliya, Younghee Lee, Hyung-Joo Kwonprobe were synthesized by Genotech (Daejeon, Korea). 10 µL of GoTaq Probe qPCR Master Mix (Catalog No. A6101, Promega) was added to 10 µL of the reaction mixture containing 125 nM each of the forward and reverse

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